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31 votes
a scientist proposed that the 32p label was entering pe molecules by direct exchange (swapping phosphate groups with those found in solution) and not through synthesis of new pe by bacterial cells. what experimental modification can show this is not the case?

User Andrei Pozolotin
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1 Answer

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18 votes

The experimental modification that can show this is not the case, is by measure the rate of incorporation of 32PO43- into a cellular PE since bacterial synthesis and phosphate exchange should have different rates.

What are bacteria?

Microscopic, single-celled organisms called bacteria exist. Bacteria are found almost everywhere in the globe.

Some species can survive in environments with high pressure and temperatures. In fact, it's thought that there are more bacterial cells within the body than there are human cells.

Staphylococcus aureus, Pesudomonas maltophilia, Thiobacillus novellus, streptococcus pyrogens, streptococcus pneumonia, Escherichia coli and Clostridium kluyveri are a few examples.

Therefore, The experimental modification that can show this is not the case, is by measure the rate of incorporation of 32PO43- into a cellular PE since bacterial synthesis and phosphate exchange should have different rates.

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User Jonny Waffles
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