Question

Continuous Diafiltration Assume the concentration of protein, C 0,1 =1.0gm/ liter, and rejection coefficient, σ 1=0.8, and glucose concentration C 0,2=1.0gm/liter, and it's rejection coefficient, σ 2=0.2. The original solution volume, V 0 =100 liter. The flow rate, Q=500 liters /hr. Plot the protein concentration, protein purity, and protein recovery with the time.

Answer 1

In continuous diafiltration, the protein concentration, purity, and recovery can be plotted over time using the given information. The concentration of protein can be calculated using the rejection coefficient. Protein purity is determined by the protein concentration compared to the total solution concentration. Protein recovery is calculated using the protein concentration, original solution volume, and flow rate.

Step-by-step explanation:

In continuous diafiltration, the concentration of protein, protein purity, and protein recovery can be plotted over time. The protein concentration is determined by multiplying the original concentration by the rejection coefficient:

Protein concentration = C0,1 * σ1

To calculate protein purity, divide the protein concentration by the sum of the protein and glucose concentrations:

Protein purity = Protein concentration / (Protein concentration + Glucose concentration)

Finally, protein recovery is calculated by multiplying the protein concentration by the original solution volume and dividing by the flow rate:

Protein recovery = (Protein concentration * V0) / Q