Question

Every year, millions of children go blind because of vitamin A deficiency. Humans cannot

produce vitamin A, thus they must consume vitamin A or a vitamin A precursor such as beta-
carotene. You want to create a yeast strain that contains the entire beta-carotene pathway
so that you can study the biochemistry of beta-carotene and vitamin A synthesis. You
discovered a yeast strain that contains the majority of the enzymes required for beta-carotene
production.
Suggest a technique you could use to confirm that you have the correct BCMO
protein in these yeast cells. Provide reasons for your choice and describe its
principle in detail.

Answer 1

One technique that can be used to confirm the presence of the correct BCMO protein in yeast cells is Western blot analysis.

Step-by-step explanation:

A technique that could be used to confirm the presence of the correct BCMO protein in yeast cells is Western blot analysis. This technique involves separating proteins by gel electrophoresis, transferring them onto a membrane, and then detecting the target protein with a specific antibody. In this case, an anti-BCMO antibody would be used to determine if the correct BCMO protein is present in the yeast cells.