Final answer:
The amount of Binding Buffer II necessary to dissolve a 156 mg gel fragment cannot be precisely determined without a specific protocol, which typically dictates the volume rather than the mass. For the DNA sample preparation, 2 µl of a 0.5 µg/µl DNA stock solution, 1 µl of 10 x TAE buffer, and 7 µl of water are needed to prepare a 10 µl sample containing 1 µg of DNA with 1 x TAE buffer.
Step-by-step explanation:
To answer the first part of your question, if you have excised a gel fragment weighing 156 mg, the amount of Binding Buffer II required to dissolve the gel will depend on the protocol provided by the particular kit or method you are using. Binding buffers are usually used in excess compared to the gel weight to ensure complete dissolution. However, without the specific protocol, it is not possible to give an exact measurement in mg. Generally, binding buffers are not measured in mg but in ml, and you would need to consult the protocol for the appropriate volume to use relative to your gel weight.
For the second part of your question regarding the preparation of a DNA sample on an agarose gel with a concentration of 1 µg in a 10 µl sample using a 0.5 µg/µl DNA stock solution and 10 x TAE buffer: To prepare this, you would use 2 µl of the DNA stock solution to get 1 µg of DNA, 1 µl of the 10 x TAE buffer to achieve a 1 x TAE concentration in your final sample, and 7 µl of water to bring the final volume to 10 µl.