Question

Tof1 has a few homologs in other organisms. Use the Align function on the Uniprot

webpage to learn about these homologs:
S. cerevisiae Tof1 P53840
C. thermophilum Tof1 G0S825
S. pombe Swi1 Q9UUM2
D. melanogaster Timeout Q8INH7
Homo sapiens Timeless Q9UNS1
You now need to perform a multiple sequence alignment on these homologs.
Multiple sequence alignments can be a powerful way of identifying functionally
important (and therefore conserved) regions of the protein. Use Uniprot to access the
amino acid sequences in FASTA format and then perform the multiple sequence
alignment using the align function of Uniprot.
In the top bar sequentially copy and paste the Uniprot IDs (after you have pasted a
recognized identifier it will clear the bar to await the next identifier and register the
protein details in the box below). When it has registered all 5 click run align and wait
for the alignment to complete. Then click on the link to see your alignment.
Download your alignment and then include the following in your submission:
A) Sequence alignment (the text of the downloaded file)
B) How many residues of the Yeast Saccharomyces cerevisiae protein are
completely conserved (indicated by dark purple) how may residues are similar
(indicated by light purple) and how many are partially similar (indicated by light
shading)?

Answer 1

To perform a multiple sequence alignment on the homologs of the Tof1 protein, use the Align function on the Uniprot webpage. Analyze the downloaded alignment to determine the number of conserved, similar, and partially similar residues in the Yeast Saccharomyces cerevisiae protein.

Step-by-step explanation:

To perform a multiple sequence alignment on the homologs of the Tof1 protein, you can use the Align function on the Uniprot webpage. Start by copying and pasting the Uniprot IDs of the homologs (P53840, G0S825, Q9UUM2, Q8INH7, Q9UNS1) sequentially into the top bar. After pasting each ID, it will clear the bar for the next one. Once all 5 IDs are registered, click the 'Run align' button and wait for the alignment to complete. Click on the link to see the alignment and download it for submission. For the second part of the question, you will need to analyze the downloaded alignment to determine the number of completely conserved, similar, and partially similar residues in the Yeast Saccharomyces cerevisiae protein. The alignment will indicate conserved residues with dark purple, similar residues with light purple, and partially similar residues with light shading.