Question

Sanger sequencing uses which of the following to determine the nucleotide sequence by terminating replication?

A) Primers
B) dNTPS
C) ATP
D) ddNTPs

Answer 1

Sanger sequencing uses D)ddNTPs to terminate replication and determine the nucleotide sequence.

Step-by-step explanation:

The Sanger sequencing method uses OPTION D :ddNTPs to determine the nucleotide sequence by terminating replication.

ddNTPs, or dideoxynucleotides, are monomers missing a hydroxyl group (-OH) at the site where another nucleotide usually attaches to form a chain. When a ddNTP is randomly incorporated into the growing complementary strand during replication, it terminates the process. These terminated strands, labeled with molecular beacons, form a ladder of differing sizes when subjected to gel electrophoresis.