Final answer:
The Declaration of Independence led to political unity, established a clear aim for independence from Britain, and symbolized a declaration of war against British rule, forming the basis for new governmental structures. Option B is the correct answer.
Step-by-step explanation:
The real and practical implications of the Declaration of Independence for the colonies at the time included manifesting a political unity among the colonies, a definitiveness of purpose in seeking independence from Britain, and a confrontation with the British monarchy that was tantamount to a declaration of war. The declaration acted as a unifying political statement that articulated Enlightenment principles and justified the colonies' intent to form a sovereign nation apart from British rule.
While it did not immediately solve economic or regional differences, it did lead to the formation of new governmental structures and addressed political and economic issues that had been problematic under British governance.