Final answer:
When two parents with the recessive genotype aa for single comb in chickens mate, the chance that their offspring will also have a single comb is 100%.
Step-by-step explanation:
In the scenario provided, both the mother and father cha-cha chickens have the genotype aa for a single comb, which is a recessive trait. This means each parent only has recessive alleles to pass to their offspring. When two parents with the genotype aa mate, all of their offspring will also have the genotype aa, as the offspring receive one allele from each parent. Since the single comb is expressed when two recessive alleles are present, the percent chance that the offspring will have a single comb is 100%. There are no dominant alleles from either parent to mask the recessive single comb phenotype.