Final answer:
The addition of the sixth enzyme to pyruvate with Phosphate and ADP during glycolysis creates phosphoenolpyruvate, which then yields pyruvate and ATP, catalyzed by pyruvate kinase.
Step-by-step explanation:
When you add the sixth enzyme to pyruvate with Phosphate and ADP, it makes phosphoenolpyruvate (PEP), which then transfers its phosphate group to ADP, producing pyruvate and ATP. This is the last step of glycolysis and is catalyzed by pyruvate kinase. When you add the final enzyme, pyruvate kinase, the products of the reaction are pyruvate and ATP.
The enzyme pyruvate kinase catalyzes the transfer of a phosphate from PEP to ADP. This reaction not only produces pyruvate but also generates one molecule of ATP per PEP, resulting in a net gain of two ATP molecules from the initial glucose molecule when considering the overall glycolytic pathway.