Question

in 1964, nirenberg and leder used the triplet binding assay to determine specific codon assignments. a complex of which of the following components was trapped in the nitrocellulose filter? (circle all that apply). a. synthetic mrna b. dna c. anti-codons d. radioactive amino acids e. large ribosomal subunits f. small ribosomal subunits

Answer 1

In the triplet binding assay, Nirenberg and Leder managed to trap a complex on the nitrocellulose filter which included synthetic mRNA, tRNA anti-codons, and radioactive amino acids, enabling the identification of specific codon assignments.

Therefore, the correct options are: a). synthetic mrna c). anti-codons and d). radioactive amino acids

Step-by-step explanation:

In 1964, Nirenberg and Leder utilized the triplet binding assay to determine the specific codon assignments. In their experiment, a complex formed by the ribosome, synthetic mRNA, and the appropriate tRNA with a bound amino acid was trapped on the nitrocellulose filter.

When this complex including the synthetic mRNA (which represented the codon), the tRNA anti-codons, and the radioactive amino acids was created, it was large enough to be retained by the filter, thus allowing the identification of which amino acid corresponded to which mRNA codon.

However, DNA, and both the large and small ribosomal subunits were not part of what was trapped in the filter in this specific assay.