Question

which of the following processes is the best way to determine whether alternative splicing of a given gene occurs? compare the size of the dna within the given gene and a similar gene in a related organism. isolate mrna from the given gene and compare the sequences. compare the sequences of the given gene and a gene known to undergo alternative splicing. isolate the primary transcripts made from the given gene and compare the sequences.

Answer 1

The most effective method to check for alternative splicing in a gene is to isolate mRNA and compare the sequences to see the expressed variants.

Step-by-step explanation:

The best way to determine whether alternative splicing of a given gene occurs is to isolate mRNA from the given gene and compare the sequences. Alternative RNA splicing is a process where different combinations of exons (and sometimes introns) are combined to form the mRNA, leading to the production of various protein products from a single gene.

This aspect of gene expression is significant in eukaryotic organisms, and it is estimated that up to 70 percent of genes in humans can undergo alternative splicing, leading to multiple proteins being expressed from a single gene. Unlike DNA sequence comparison, examining mRNA provides insight into the actual expressed sequences after post-transcriptional modifications, including splicing.