Final answer:
Ion-exchange chromatography is a mode of separation for chromatography that involves the separation of molecules based on their charge. It uses a stationary phase with charged sites to attract and retain ions with opposite charges. This allows for the separation of different ions present in the sample.
Step-by-step explanation:
Ion-exchange chromatography is a mode of separation for chromatography that involves the separation of molecules based on their charge. It works by using a stationary phase with charged sites that attract and retain ions with opposite charges. When a sample is injected into the mobile phase, the ions in the sample interact with the charged sites on the stationary phase, causing them to be separated based on their charge.
The ions with stronger interactions with the stationary phase will move slower, while the ions with weaker interactions will move faster. This allows for the separation of different ions present in the sample.