Final answer:
Mass spectrophotometry (MS) can be coupled with High Performance Liquid Chromatography (HPLC), and this is true. The LC/MS technique combines the separation capabilities of HPLC with the analysis power of MS, commonly using ionization methods like ESI or APCI for detecting and quantifying compounds with high precision.
Step-by-step explanation:
Mass spectrophotometry (MS) can indeed be coupled with High Performance Liquid Chromatography (HPLC), and the statement is true. The coupling of these two techniques is commonly referred to as LC/MS or LC/MS/MS. High Performance Liquid Chromatography (HPLC) is a method used for separating the components of a mixture by passing the mixture through a stationary phase at high pressure.
The separated components are then analyzed by Mass Spectrometry (MS), which involves ionizing chemical compounds to generate charged molecules and measuring their mass-to-charge ratios. This combination enhances the capabilities of both techniques, providing detailed information about the composition, structure, and abundance of substances within complex mixtures. The use of electrospray ionization (ESI) or atmospheric pressure chemical ionization (APCI) are common methods for ionization prior to MS detection. LC/MS has become an invaluable tool in various fields, including drug discovery and development (DDD), where it aids in the high-throughput analysis of drugs and their metabolites with high sensitivity and specificity.