Final answer:
The greatest appreciable changes in ROM occur with static stretching over several cycles, which affects cytoskeletal tension and actomyosin cross-bridges.
Step-by-step explanation:
Research suggests the greatest appreciable changes in range of motion (ROM) occur with static stretching when it is maintained for several cycles. During a stepwise stretch, cytoskeletal tension increases, but upon the removal of the stretch, the tension decreases below baseline levels. Cellular responses to a constant stretch include strain-stiffening, which is attributed to the nonlinear stretching of cytoskeletal filaments. Conversely, strain-softening after stretch cessation is likely due to the inelastic unbinding or unfolding of cytoskeletal crosslinks and actomyosin cross-bridges. This process is influenced by the homeostatic contractility of cells, which is adjusted through staged calcium-dependent processes involving depolymerization and rebuilding of actin filaments. This dynamic adaptation allows cells to exert different mechanical tractions during and after the stretching process, highlighting two distinct mechanisms by which cells respond to stretch.