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What technique would be most appropriate to use to observe the movements of condensed chromosomes during cell division?

A) light microscopy
B) scanning electron microscopy
C) transmission electron microscopy
D) confocal fluorescence microscopy
E) super-resolution fluorescence microscopy

User Ende Neu
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1 Answer

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Final answer:

The best technique for observing the movements of condensed chromosomes during cell division is confocal fluorescence microscopy, as it allows for the real-time visualization of live cell dynamics with the aid of fluorescent stains.

Step-by-step explanation:

To observe the movements of condensed chromosomes during cell division, the most appropriate technique would be D) confocal fluorescence microscopy. This type of microscopy allows scientists to visualize live cells and their internal structures in detail, by using fluorescent stains to highlight specific components like chromosomes. It provides the ability to generate sharp images of structures within the thick cellular environment without interference from out-of-focus light, which is essential for watching dynamic processes such as chromosome movement during cell division.

Confocal fluorescence microscopy overcomes the limits of magnification and resolution common to all forms of light microscopy, which typically have a maximum magnification of 1200X and resolution of 2 mm. In contrast to electron microscopy, confocal microscopy permits the observation of live cell dynamics, making it an ideal choice for visualizing chromosome movements in real time.

User Hellion
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