Final answer:
Model organisms like mice and rats are critical in medical research but have limitations, including species differences, lack of suitable models for certain conditions, and the potential non-transferability of genetic manipulations or disease responses.
Step-by-step explanation:
The limitations in generalizing findings from model organisms to human biology center on the biological differences between species. Although model organisms, such as mouse and rat, are invaluable in medical research for studying diseases and potential treatments, there are key differences in genetics, physiology, and response to treatments that may not accurately represent human biology. Not all discoveries in model organisms are directly translatable to humans because of these differences; some diseases lack suitable animal models, and genetic manipulations may not be possible in some pathogens. Moreover, while computer simulation models of biological systems are useful, they require experimental validation and may not fully capture complex biological interactions without data from living organisms or tissues.