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Tra locus: Which protein directs the TraY/I/M-DNA complex to the T4SS channel? (Note: It was not covered in the lectures, but TraT/Y complex also contains TraM.)

User Ryuzaki L
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Final answer:

The question is about the protein that guides the TraY/I/M-DNA complex to the T4SS during bacterial plasmid conjugation. Although not detailed in the provided literature, coupling proteins like Trad are recognized for guiding the relax some to the T4SS channel.

Step-by-step explanation:

The student is asking about the transfer and replication (Tra) locus, specifically related to the conjugative transfer of DNA in bacteria. The question involves the component of the Tra system that directs the transfer of DNA-carrier complexes to the type IV secretion system (T4SS). While the literature provided does not detail this exact mechanism, it's important to understand proteins like Tram, Tray, and TraI are part of the relax some complex involved in bacterial plasmid conjugation. This complex prepares the plasmid DNA for transfer by introducing a nick and binding to the DNA, effectively forming a complex that can be recognized by the T4SS. The relax some is typically guided to the T4SS channel by coupling proteins like Trad in the F-plasmid system, which interact with both the relax some and the T4SS, facilitating the transport of DNA during bacterial conjugation.

User Henry Tseng
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