Final answer:
The question deals with the biology of cell division, focusing on DNA replication during the S phase of the cell cycle and chromosome behavior during mitosis. An observation of labeled chromosomes at metaphase would signify the presence of incorporated nucleotides following DNA replication.
Step-by-step explanation:
The subject of the question is Biology, specifically focusing on the cell cycle and mitosis. During the S Phase, each chromosome is replicated to form two identical sister chromatids connected at the centromere. During mitosis, these chromosomes will pass through the phases prophase, prometaphase, metaphase, anaphase, and telophase. At metaphase, chromosomes align at the metaphase plate. After mitosis, each daughter cell receives an identical set of chromosomes.
An autoradiographic image showing the chromosomes during the metaphase would reveal the locations of the labeled nucleotides incorporated during the S phase. If a cell went through one S phase labeling with 3H-thymidine just before observation, we would expect to see the label on all sister chromatids, since thymidine is a component of the DNA molecule and would be incorporated into all the DNA replicated during S phase.