Final answer:
An exact calculation of the distance between two holes on a flute based solely on the difference in frequency is complex due to various interacting factors; therefore, an answer would require additional data or a detailed acoustic model.
Step-by-step explanation:
The question pertains to the physical principles of sound waves and their interaction with musical instruments, specifically a flute. It involves the concept of resonating air columns that create different frequencies based on the length of the air column. To find out how far apart the two holes should be to play two frequencies that are 300.0 Hz apart, we would need to use the formula for the fundamental frequency of an open pipe, f = v/(2L), where f is the frequency, v is the speed of sound, and L is the length of the tube or air column. However, since the actual distance between the holes depends on the complex interaction of the changing resonant frequencies of the air column as holes are opened and closed, as well as acoustic impedance and other factors, an exact calculation from this principle alone is not straightforward and usually requires additional empirical data or a more detailed acoustical analysis model.