Final answer:
Hajslova et al. were able to more effectively measure δ9-THC and other trace cannabinoids in a CBD oil sample with a very large CBD peak during LC-MS analysis. The correct option is B.
Step-by-step explanation:
Hajslova et al. improved the measurement of cannabinoids in a CBD oil sample by diluting the CBD oil sample. This method was effective in mitigating the issue of the CBD peak overwhelming the measurement of trace cannabinoids like δ9-THC. Dilution helped in correctly identifying and quantifying trace cannabinoids as it reduced the CBD concentration, preventing interference and enabling better resolution and sensitivity during LC-MS analysis. It allows for better resolution and detection of the trace cannabinoids by LC-MS. This method ensures accurate measurement and prevents overestimation of the concentration of the parent compound.
Improved chromatographic separation and sensitivity are crucial, especially in the field of bioanalysis during drug discovery and development (DDD), in which reversed-phase liquid chromatography is commonly employed to separate small molecules. Ultimately, dilution further helps in eliminating the possibility of ion suppression or enhancement effects caused by co-elution of compounds when employing mass spectrometric detection, and it simplifies the challenge of quantifying cannabinoids with high precision.