Question

In what order are the GTFs added in transcription initiation?

Answer 1

The general transcription factors (GTFs) are added in a specific order during transcription initiation: TFIID, TFIIB, TFIIE and TFIIF, and TFIIH.

Step-by-step explanation:

The general transcription factors (GTFs) are added in a specific order during transcription initiation. The order of GTF addition is as follows:

1. TFIID: TFIID is the first GTF to bind to the TATA box in the promoter region.
2. TFIIB; TFIIE and TFIIF:: TFIIB binds to TFIID and helps recruit other initiation factors and RNA polymerase II.
3. TFIIE and TFIIF bind to the preinitiation complex and stabilize it.
4. TFIIH: TFIIH is the final GTF to bind and helps unwind DNA and phosphorylate RNA polymerase II.

Once all the GTFs are bound, the transcription initiation complex is formed and RNA polymerase II can start transcribing the DNA into mRNA.