Final answer:
The promoter of T7 RNAP is a 19-nucleotide long hairpin structure. T7 RNA polymerase recognizes this promoter and synthesizes RNA in the 5' to 3' direction. Eukaryotic promoters, such as those recognized by RNA Polymerase II, contain a TATA box motif.
Step-by-step explanation:
The promoter of T7 RNAP (T7 RNA polymerase) is 19-nucleotide long and forms a hairpin structure. This promoter sequence needs to be present upstream of the DNA template sequence in the cloning vector to enable successful transcription.
Figure 7.4 shows that T7 RNA polymerase recognizes a hairpin-shaped promoter and synthesizes RNA in the 5' to 3' direction, where each T is replaced by U in the RNA sequence.
The promoter of RNA Polymerase II in eukaryotes, which is responsible for transcribing protein-coding genes, typically contains a TATA box motif and additional short upstream sequences.