Question

In order to clone a gene mammalian gene by complementation of a yeast temperature-sensitive mutation you would use:

a. a genomic library
b. PCR
c. a cDNA library
d. either a or c
e. none of the above

Answer 1

The correct answer is a genomic library.

Step-by-step explanation:

The correct answer to the question is a. a genomic library.

In order to clone a mammalian gene by complementation of a yeast temperature-sensitive mutation, a genomic library would be used. Genomic libraries are made by cloning genomic fragments from one organism into plasmid vectors or bacteriophages. These libraries contain a complete or nearly complete copy of an organism's genome, allowing researchers to create large quantities of each fragment for DNA sequencing and gene function studies.