Question

What method can be used to functionally inactivate a gene without altering its sequence?

a. gene knockout
b. RNA interference
c. dominant negative mutation
d. b and c
e. all of the above

Answer 1

To functionally inactivate a gene without altering its sequence, RNA interference and dominant negative mutations can be used. These methods do not involve changing the gene's DNA but rather affect the gene's expression or function.

Step-by-step explanation:

The method that can be used to functionally inactivate a gene without altering its sequence is RNA interference (RNAi) and dominant negative mutation. RNAi is a process that allows cells to control the amount of gene expression, typically by causing the destruction of specific RNA molecules. On the other hand, a dominant negative mutation results in a gene product that interferes with the normal function of the wild-type gene product within the same cell, often by being a competitive inhibitor.