Final answer:
Topoisomerases, particularly topoisomerase II or DNA gyrase, and helicase are the main proteins that assist in resolving the supercoiled nature of DNA during replication, with SSBs maintaining the strands apart.
Step-by-step explanation:
The proteins that help remove DNA from its supercoiled nature during DNA replication are called topoisomerases. Specifically, topoisomerase II, also known as DNA gyrase, relaxes the supercoiled DNA. This enzyme makes precise cuts in the DNA's phosphate backbone, allowing the DNA to unwind and relieve tension before resealing the breaks. An additional protein known as helicase separates the DNA strands at the origin of replication. Single-stranded binding proteins (SSBs) then bind to the single-stranded DNA to prevent it from reannealing into a double helix, facilitating replication.