Final answer:
Reducing the incubation time for tube 5 to 30 minutes will result in an incomplete enzymatic reaction and a lower amount of product formed.
Step-by-step explanation:
If the incubation time for tube 5 is reduced to 30 minutes, the enzymatic reaction will not have sufficient time to occur completely.
Pepsin is an enzyme that breaks down proteins, and in this case, it is reacting with BAPNA (a synthetic substrate) in a pH 7.0 buffer. The standard incubation time for this reaction is 3-4 hours at 37°C. If the incubation time is reduced to only 30 minutes, the reaction will be incomplete, leading to a lower amount of product formed.