Final answer:
In complementation testing in yeast, diploid cells are not required.
Step-by-step explanation:
In complementation testing in yeast, all of the following are required except diploid cells. In complementation testing, different mutants with the same phenotype are combined and tested to see if they complement each other, indicating that they have mutations in different genes. Diploid cells, which contain two copies of each gene, are not required for this type of testing.