Final answer:
Simultaneously with the removal of RNA primers, DNA polymerase I fills in the resulting gap with deoxyribonucleotides and DNA ligase then seals the gaps between DNA fragments.
Step-by-step explanation:
When RNA primers are removed by the 5'—>3' exonuclease activity of DNA polymerase I during DNA replication, the gap left by the removal of the RNA primer is immediately filled in with deoxyribonucleotides. This process is critical for the completion of the lagging strand synthesis, where short fragments of DNA known as Okazaki fragments are created. DNA polymerase I uses the existing DNA strand as a template to add the correct nucleotides in place of the RNA primer. Soon after this, the DNA ligase enzyme seals the gaps between these newly-synthesized DNA fragments by creating phosphodiester bonds, ensuring a continuous DNA strand.