Question

Place the steps in base excision repair in the correct order.

1 - Damaged base is removed by cleavage of glycosidic linkage attaching it to deoxyribose of backbone.
2 - Baseless deoxyribose phosphate in site is excised by a specialized endonuclease and a DNA polymerase.
3 - DNA glycosylase recognizes alteration in DNA.
4 - The strand is sealed by DNA ligase III.
5 - Gap is filled by DNA polymerase β; it inserts a nucleotide complementary to the undamaged strand. a. 1 - 3 - 2 - 5 - 4
b. 3 - 1 - 2 - 4 - 5 751
c. 1 - 3 - 2 - 4 - 5
d. 3 - 2 - 1 - 5 - 4
e. 3 - 1 - 2 - 5 - 4

Answer 1

The steps in base excision repair are: 1. DNA glycosylase recognizes alteration in DNA. 2. Damaged base is removed by cleavage of glycosidic linkage attaching it to deoxyribose of backbone. 3. Baseless deoxyribose phosphate in site is excised by a specialized endonuclease and a DNA polymerase. 4. Gap is filled by DNA polymerase β; it inserts a nucleotide complementary to the undamaged strand. 5. The strand is sealed by DNA ligase III.

Step-by-step explanation:

1. DNA glycosylase recognizes alteration in DNA.
2. Damaged base is removed by cleavage of glycosidic linkage attaching it to deoxyribose of backbone.
3. Baseless deoxyribose phosphate in site is excised by a specialized endonuclease and a DNA polymerase.
4. Gap is filled by DNA polymerase β; it inserts a nucleotide complementary to the undamaged strand.
5. The strand is sealed by DNA ligase III.