Question

In in vitro replication systems using cellular extracts, how can specific proteins be removed from the preparation for the purpose of analyzing how their absence adversely affects the replication process?

a. targeted denaturation by altered pH
b. targeted denaturation by altering the temperature
c. precipitation of the protein that is to be deleted with specific antibodies to remove it from solution
d. precipitation of the protein that is to be deleted with ammonium sulfate to remove it from solution
e. precipitation of the protein that is to be deleted with RNA to remove them from solution

Answer 1

Specific proteins can be removed from cellular extract in in vitro replication systems by targeted denaturation or precipitation using antibodies or ammonium sulfate.

Step-by-step explanation:

In in vitro replication systems using cellular extracts, specific proteins can be removed from the preparation for the purpose of analyzing how their absence affects the replication process through targeted denaturation by altered pH or temperature, and precipitation of the protein with specific antibodies or ammonium sulfate to remove it from solution.