Final answer:
FEN-1 and DNA polymerase δ are responsible for the removal of RNA primers and short DNA segments added during DNA replication, with the assistance of DNA ligase to seal the fragments.
Step-by-step explanation:
The enzymes responsible for removing the RNA primer and short DNA segments added by eukaryotic DNA polymerase α are FEN-1 (Flap Endonuclease 1) and DNA polymerase δ. These two components work together to process the Okazali fragments during DNA replication.
FEN-1 functions to remove the 'flaps' of nucleic acid from the 5' ends of the fragments that are often displaced by the polymerase. Once the RNA nucleotides are removed by the exonuclease activity, DNA polymerase δ catalyzes the replacement of RNA with DNA nucleotides. Following this process, DNA ligase comes into play to link the DNA fragments together, creating a continuous strand.