Final answer:
Under those conditions, the Acholeplasma culture in flask B would deplete nutrients, accumulate waste, and experience reduced or ceased growth without fresh media.
Step-by-step explanation:
If you were to maintain the Acholeplasma culture of part B under the conditions described for an extended period of time, it is likely that the bacterial cells would eventually exhaust the available nutrient supply and accumulate toxic metabolites. This process would lead to a decline in the population growth until the bacteria entered a stationary phase or died out if not subcultured to fresh media. Consistently providing fresh nutrients and optimal growing conditions is crucial for sustaining bacterial cultures in the long term, much like the scenario described in part E for the E. coli population where additional nutrient broth is continuously supplemented.