Question

12. A 70-year-old patient with complaints of high grade fever, chills, shivering, and arthralgia was sent to the laboratory for blood film examination. The laboratory technologist took a blood sample and found trophozoite stage of P. vivax. However, he wanted to see the effect of the parasite on the red blood cells. What is the most likely method used for the examination of the blood cells? (A) Examination of RDT (B) Examination of thin blood film C Examination of thick blood film (D) Examination of Fluorescent microscopy 33. A 25-year-old patient, living in a malaria endemic area, was sent to a laboratory for rapid diagnostic test of malaria since the microscope was non-functional. The laboratory technologist observed a positive test result on HRP2 and lactate dehydrogenase lines. What parasite is the most likely diagnosed in the test? (A) Plasmodium vivax (B) Plasmodium ovale 34. A 50-year-old febrile patient was sent to a laboratory for blood film examination. The laboratory technologist examined 100x oil immersion fields using a high power objective and found 80 trophozoite stage of Plasmodium vivax What is the most appropriate way to report this finding using a plus sign reporting system? (B) +2 (A) +1 (C) +3 (D) +4 35. Blood specimen is received at serology laboratory from a patient suspected of jaundice for. hepatitis serology. Serologic assays demonstrated the following result. Result Positive Sino Serological Marker 1 HBsAg Anti-HBs Anti-HBc Anti-HBe (C) Plasmodium malariae (D) Plasmodium falciparum 2 3 4 Negative Positive Negative What is the most likely interpretation of this result? (A) Immune due to infection (B) Immune due to vaccination (C) Acute infection (D) Chronic infection​

Answer 1

The most likely method used for the examination of the blood cells to observe the effect of the parasite on red blood cells is: (C) Examination of thick blood film.

Based on the positive test result on HRP2 and lactate dehydrogenase lines, the most likely diagnosed parasite in the rapid diagnostic test is: (D) Plasmodium falciparum.

Using a plus sign reporting system for the finding of 80 trophozoite stage of Plasmodium vivax would most likely be reported as: (A) +1.

The most likely interpretation of the serologic assay results provided (HBsAg positive, Anti-HBs negative, Anti-HBc positive, Anti-HBe negative) is: (C) Acute infection.

12 Thick blood film examination allows for better detection and visualization of the malaria parasite within red blood cells, enabling a more detailed assessment of its stages and effects.

33 These markers are often associated with Plasmodium falciparum infection in rapid diagnostic tests for malaria.

34 The plus sign reporting system quantifies the density of malaria parasites in blood films, and +1 typically corresponds to 1-10 parasites per high power field at 1000x magnification.

35 This pattern of results indicates the presence of HBsAg (surface antigen of the hepatitis B virus), presence of Anti-HBc (antibodies to the core antigen of the virus), and absence of Anti-HBs (antibodies to the surface antigen, suggesting lack of immunity). The absence of Anti-HBe (antibodies to the e antigen) can be seen in acute infection or during the window phase of the infection.