Final answer:
Spanish colonization affected Native American tribes in Florida through the imposition of the exploitative encomienda system, which used Native workers in mines and plantations.
Step-by-step explanation:
One way Spanish colonization affected Native American tribes in Florida was through the imposition of the encomienda system. Under this system, Spanish authorities assigned Native workers to mine and plantation owners in exchange for protection and the teaching of Christianity. However, the encomienda system exploited native workers and led to their mistreatment. Eventually, this system was replaced by the repartimiento, which also required Native towns to supply labor for Spanish overlords.