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In a dividing cell in its metaphase, the chromosomes would be positioned at the center, aligned along the cell's equator, and the spindle fibers would connect to the centromeres of the chromosomes.

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In metaphase, sister chromatids line up along the cell's equator, connected by spindle fibers to their centromeres. Centromeres serve as attachment points for spindle fibers and play a key role in chromatid separation during cell division. This stage is visually identifiable by the alignment of highly condensed chromosomes along the metaphase plate.

Step-by-step explanation:

During metaphase, a critical phase within both mitosis and meiosis, sister chromatids align themselves along the equator or metaphase plate of the cell. This alignment is orchestrated by the spindle apparatus, which is composed of microtubules emanating from the cell poles. Each chromosome is connected to spindle fibers via its centromeres. The attachment sites on the centromeres are the kinetochores, to which the spindle fibers attach, ensuring that each sister chromatid is pulled to opposite poles when the cell proceeds to anaphase. During Metaphase I of meiosis, homologous chromosome pairs, not sister chromatids, line up along the metaphase plate.

It is crucial to understand that the term sister chromatids refer to the duplicated forms of a single chromosome that are still attached to each other. The centromeres act as pivotal regions for the attachment and eventual separation of these sister chromatids. At the metaphase stage, chromosomes reach their highest level of condensation, making them easily observable under a microscope.

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