Question

Describe the procedure to prepare and view a spider's web under the microscope. Include (1) the steps to prepare a wet mount of the slide, (2) the process to focus the web under low power, and (3) the process to focus the web under high power.

Answer 1

To prepare and view a spider's web under the microscope, follow these steps: 1) Prepare a wet mount by placing a section of the web on a slide with liquid. 2) Focus the web under low power by adjusting the stage and using the fine focusing knob. 3) Switch to high power and adjust the focus to obtain a clear view of the web.

Step-by-step explanation:

1. To prepare a wet mount of a spider's web, place a drop of liquid (such as water or stain) on a slide. Carefully transfer a section of the web onto the liquid using forceps. Place a coverslip on top of the specimen.
2. To focus the web under low power, start with the lowest magnification objective lens (usually 4x). Use the coarse focusing knob to move the stage up or down until the web comes into focus. Then, use the fine focusing knob to sharpen the image.
3. To focus the web under high power, switch to a higher magnification objective lens (e.g., 40x). Use the coarse focusing knob to move the stage up or down until the web is approximately in focus. Then, use the fine focusing knob to further adjust the focus and obtain a clear view of the web.