Final answer:
The troubles between the Muscogee and Hernando de Soto's men arose due to the Spaniards' encroachment on Muscogee territory and resources, fueled by Spain's imperial ambitions and the quest for gold, which led to conflict and devastation from introduced diseases. Hence, the correct answer is option 2.
Step-by-step explanation:
The troubles between the Muscogee people and Hernando de Soto and his men primarily stemmed from encroachment on Muscogee territory and resources. De Soto was a Spanish conquistador who led expeditions throughout the southeastern United States from 1539 to 1542, seeking gold. These explorations often led to conflicts as the Spanish arrived with intentions to claim land and search for precious materials, viewing the New World as ripe for conquest and indigenous peoples as obstacles to their goals of wealth and expansion.
The Spanish brought with them diseases which devastated local populations, and their relentless quest for gold pitted them against the native groups, who resisted these invasions. Additionally, despite any potential gains, the expeditions of De Soto failed to find the fabled riches, ultimately returning to Mexico City considerably diminished in number and without the anticipated wealth.
In summary, the conflicts were a consequence of Spanish imperial ambitions clashing with the indigenous populations who defended their homelands against foreign intrusion and exploitation. It was a clear case of colonial aggression where the Muscogee people resisted the Spaniards' attempts to appropriate their lands and resources, leading to the aforementioned troubles.