Final answer:
The LaSalle expedition made the Spanish realize the urgency to colonize Texas, fearing they might lose it to the French; it was a catalyst for Spanish action to secure their territorial claims.
Step-by-step explanation:
The LaSalle expedition had a significant impact on the Spanish in terms of their claims in North America. This French exploration led by LaSalle heightened the Spanish concern over their territorial claims, specifically regarding Texas. The correct impact of the LaSalle expedition on the Spanish was that It made the Spanish realize they needed to colonize Texas or risk losing it to the French. The fear of losing territory spurred the Spanish to take action to solidify their presence in regions that were being eyed by other European powers, such as the French.