Question

If you have a solution of substrate with a concentration of 2.5 mM, what is the concentration of the substrate if you make a 5-fold dilution? What is the concentration if you make a 25- fold dilution? (Note fill in two answers here, there are two questions)_____

Answer 1

The concentration of the substrate after a 5-fold dilution is 0.5 mM, and after a 25-fold dilution, it is 0.1 mM.

Step-by-step explanation:

To find the concentration of the substrate after a 5-fold dilution, we first need to understand how dilution affects concentration. Dilution involves adding solvent to a solution, which decreases the concentration of the solute. In a 5-fold dilution, the volume of the solution is increased by a factor of 5, resulting in a five times lower concentration of the solute. Therefore, the concentration of the substrate after a 5-fold dilution would be 2.5 mM / 5 = 0.5 mM.

Similarly, in a 25-fold dilution, the volume of the solution is increased by a factor of 25. Therefore, the concentration of the substrate after a 25-fold dilution would be 2.5 mM / 25 = 0.1 mM.