Question

Which of the following statements below is NOT correct regarding thin layer chromatography:

a. gloves must be worn when working with thin layer chromatography sheets
b. samples must be spotted 2cm up from the bottom of the plate
c. using pen write your initials
d. samples must be spotted on the side coated with silica(not the shiny plastic side)

Answer 1

The incorrect statement regarding thin-layer chromatography is that you should use a pen to write your initials. It's crucial to use the pencil to avoid contamination.

Step-by-step explanation:

The question pertains to the procedures and best practices for performing Thin Layer Chromatography (TLC). Among the choices provided, the statement that is NOT correct regarding thin layer chromatography is c. Using pen write your initials. When labeling a TLC plate, it's important to use a pencil instead of a pen, because the ink from the pen can interfere with the chromatography process.

For good laboratory practice and to ensure precise results in TLC:

• Gloves must be worn to protect against chemicals and avoid contaminating the TLC plate.
• Substances should be spotted on the side coated with silica, which is the correct stationary phase and not the shiny plastic side.
• It is commonly instructed to spot samples about 1cm from the bottom of the plate, not 2cm, although this distance can vary and should be clarified in lab guidelines or protocol.