Final answer:
The statement regarding glycolysis that is true is that it initially requires energy input in the form of ATP hydrolysis (Option A).
Step-by-step explanation:
The correct answer to the question regarding glycolysis is A. It initially requires energy input in the form of ATP hydrolysis.
Glycolysis is a metabolic pathway that takes place in the cytosol of cells and has two key stages. In the first stage, ATP is consumed to phosphorylate glucose and convert it to fructose-1,6-bisphosphate in preparation for the second stage. In the second stage, the energy is extracted as ATP is regenerated from ADP by the process of substrate-level phosphorylation, and not by oxidative phosphorylation, which is related to the electron transport chain and ATP synthase. Glycolysis can occur in the absence of oxygen, operating under anaerobic conditions. Therefore, it does not utilize ATP synthase (ATPase) as implied in option B, nor does it form ATP via oxidative phosphorylation as stated in option C, and it does not require the presence of oxygen, in contradiction with option D.