Question

as a cell enters mitosis the now active mpf mediates phosphorylation of many of the key proteins required for progression through prophase. what are the targets of mpf phosphorylation involved in preparation of sister chromatids for separation?

Answer 1

During mitosis, MPF targets proteins involved in the preparation of sister chromatids for separation, including those that condense chromatin, breakdown the nuclear envelope, enable spindle fiber formation, and catalyze the separation of chromatids at their centromeres.

Step-by-step explanation:

During mitosis, the active MPF (Mitosis Promoting Factor) mediates phosphorylation of key proteins involved in the preparation of sister chromatids for separation. The targets of MPF phosphorylation in this process are proteins that bind to chromatin causing it to condense and form chromatids, proteins that lead to the breakdown of the nuclear envelope, and proteins that enable spindle fiber formation. Additionally, tension in the spindle apparatus at metaphase triggers an activated separase enzyme, which causes the chromatids to separate at their centromeres.