Question

Protein X has an absorptivity of 0.4 mL . mg -1 . cm-1 at 280nm. What is the ab sorbance at 280 nm of a 2.0 mg . mL-1 solutionof protein X ?

Answer 1

The absorbance of a 2.0 mg . mL-1 solution of protein X at 280 nm is 0.8.So, the absorbance at 280 nm of a 2.0 mg . mL-1 solution of protein X is 0.8.

Step-by-step explanation:

The absorbance of a solution can be calculated using the formula:

A = εbc

Where A is the absorbance, ε is the molar absorptivity, b is the path length of the light through the sample, and c is the concentration of the solution.

In this case, the molar absorptivity (ε) for protein X at 280 nm is 0.4 mL . mg-1 . cm-1.

The concentration of the solution is 2.0 mg . mL-1. Assuming a path length of 1 cm:

A = (0.4 mL . mg-1 . cm-1) * (2.0 mg . mL-1) * (1 cm) = 0.8

So, the absorbance at 280 nm of a 2.0 mg . mL-1 solution of protein X is 0.8.