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Select the statements that describe genomic DNA libraries. a) Only includes DNA from exons

b) Useful for determining gene function
c) Developed for genome sequencing
d) DNA is synthesized from template mRNA
e) DNA fragments are cloned into vectors

1 Answer

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Final answer:

Genomic DNA libraries are collections of cloned DNA fragments representing an organism's entire genome, useful for gene function analysis and genome sequencing. These libraries include both coding and non-coding sequences and are stored within vectors such as plasmids or bacteriophages. The correct options are b), c) and e).

Step-by-step explanation:

Understanding Genomic DNA Libraries:

A genomic DNA library is a collection of cloned DNA fragments that together represent the entire genome of an organism. Within these libraries, the DNA is often stored within vectors such as plasmids or bacteriophage, enabling the replication and analysis of the DNA. Genomic DNA libraries are essential for various applications, including determining gene function, and they play a critical role in genome sequencing efforts. Unlike cDNA libraries, which are derived from mRNA and only include the expressed genes of an organism, genomic libraries contain both exonic and intronic sequences as well as regulatory elements, thus giving a comprehensive overview of an organism’s genetic blueprint.

Regarding the student's question, the correct statements that describe genomic DNA libraries are:

  • b) Useful for determining gene function
  • c) Developed for genome sequencing
  • e) DNA fragments are cloned into vectors

Statement a) is incorrect because genomic libraries include both exons and introns, as well as non-coding sequences. Statement d) is also incorrect because this describes the process of creating a cDNA library, which involves synthesizing DNA from template mRNA.

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