Final answer:
ATP synthase uses the proton gradient across the thylakoid membrane to generate ATP during photosynthesis.
Step-by-step explanation:
The generation of ATP by ATP synthase in photosynthesis is driven by a proton gradient across the thylakoid membrane. The high concentration of hydrogen ions (protons) in the thylakoid lumen creates a gradient, with the lumen having a higher concentration than the stroma. As the hydrogen ions flow through ATP synthase during chemiosmosis, the energy released allows ATP synthase to attach a third phosphate group to ADP, forming ATP.