Final answer:
Pyrosequencing is a next-generation sequencing technique that involves amplifying and sequencing DNA fragments. By adding nucleotides one at a time and detecting the release of pyrophosphate, the order of nucleotides in the DNA can be determined.
Step-by-step explanation:
Pyrosequencing is a next-generation sequencing technique used for rapid DNA sequencing. In pyrosequencing, a DNA sample is fragmented into single-strand fragments, which are then modified with DNA adapters. Each fragment is amplified and immobilized on a bead, and then placed in a well with sequencing enzymes. Each of the four nucleotides is added one after the other, and when each one is incorporated, pyrophosphate is released and emits a small flash of light that is recorded by a detector. This allows for the order of nucleotides in the DNA to be determined.