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how one can verify whether the separation of lycopene and b-carotene was successful via column chromatography.

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Final answer:

To verify the success of the separation of lycopene and b-carotene via column chromatography, you can collect fractions as they elute from the column or use a suitable detector to analyze the elution profile.

Step-by-step explanation:

In order to verify whether the separation of lycopene and b-carotene was successful via column chromatography, you can follow the progress of the separation by collecting fractions as they elute from the column or by placing a suitable detector at the end of the column.

By collecting fractions, you can visually observe the separation of the two components. If the separation was successful, you should be able to collect separate fractions corresponding to lycopene and b-carotene as they elute from the column.

Alternatively, you can use a suitable detector, such as a UV (diode-array) detector, to analyze the elution profile of the column. A chromatogram will be generated, which consists of a peak for each solute. If the separation was successful, you should see distinct peaks for lycopene and b-carotene.

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