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When does the centrosome duplicate to form the two poles of a mitotic spindle?

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Final answer:

The centrosome duplicates during interkinesis to form the two poles of a mitotic spindle during prophase II. The mitotic spindle, composed of the centrosomes and microtubules, helps separate the duplicated chromosomes during mitosis.

Step-by-step explanation:

The centrosome duplicates during interkinesis to form the two poles of a mitotic spindle. During prophase II, the centrosomes that were duplicated move away from each other toward opposite poles and new spindles are formed. The mitotic spindle is composed of the centrosomes and their emerging microtubules, which play a crucial role in separating the duplicated chromosomes during mitosis.

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