Final answer:
During DNA replication, the interlinked DNA circles can be separated by bacterial topoisomerase IV, which introduces double-stranded breaks and reseals the circular chromosomes.
Step-by-step explanation:
During DNA replication, the two interlinked DNA circles can be separated through the activity of bacterial topoisomerase IV.
This enzyme introduces double-stranded breaks into the DNA molecules, allowing them to separate from each other. The enzyme then reseals the circular chromosomes. This process is specific to prokaryotic DNA replication due to their circular chromosomes.