Final answer:
Yeast two Hybrids is a method used to detect protein-protein interactions. The two proteins that are bound in Yeast two Hybrids depend on the specific experiment being conducted. In the study of Japanese birch plantlet No.8, the E12 protein was identified as the bait, and hypothetical proteins D1 and D2 were identified as the prey proteins.
Step-by-step explanation:
Yeast two Hybrids is a method used to detect protein-protein interactions. It involves combining two hybrid proteins, one called the bait and the other called the prey, in a yeast cell. If the bait and prey proteins interact, they will activate a reporter gene, leading to a visible signal. The two proteins that are bound in Yeast two Hybrids depend on the specific experiment being conducted. The bait protein can be any protein of interest, while the prey protein can be a library of proteins to identify potential interacting partners. For example, in the study of Japanese birch plantlet No.8, the E12 protein was identified as the bait, and hypothetical proteins D1 and D2 were identified as the prey proteins.